Changes in red blood cell membrane structure in G6PD deficiency: an atomic force microscopy study.
Clin Chim Acta
; 444: 264-70, 2015 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-25748035
ABSTRACT
BACKGROUND:
Glucose-6-phosphate dehydrogenase deficiency affects over 400 million people worldwide. The hemolytic anemia in G6PD deficiency is usually triggered by oxidative stress, but the mechanism remains uncertain. We have used atomic force microscopy for studying changes in red blood cell membrane and providing new insights on the mechanism.METHODS:
G6PD activity assay and molecular genetic tests were used for molecular diagnosis. AFM was used to investigate alterations in the ultrastructure of G6PD deficient RBC membranes, the influence of different primaquine concentrations, and the protective effects of vitamin C.RESULT:
Nine variants were identified from 33 G6PD deficient individuals. AFM imaging and quantitative analysis showed that G6PD deficient erythrocytes became heterogeneous and roughness measurements of erythrocyte membranes are increased. G6PD enzyme activity and different mutations may relate with roughness parameters. Furthermore, primaquine induces an increased roughness and height of erythrocyte membrane. Meanwhile, primaquine induces damages to erythrocytes which could be prevented by vitamin C treatment in normal RBCs but not in G6PD deficient erythrocytes.CONCLUSIONS:
Our research may give valuable information about the status of G6PD deficient patients and explore the mechanism of hemolytic anemia.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estruturas da Membrana Celular
/
Membrana Eritrocítica
/
Deficiência de Glucosefosfato Desidrogenase
Limite:
Humans
Idioma:
En
Revista:
Clin Chim Acta
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China