P5-2 of rice black-streaked dwarf virus is a non-structural protein targeted to chloroplasts.
Arch Virol
; 160(5): 1211-7, 2015 May.
Article
em En
| MEDLINE
| ID: mdl-25749897
ABSTRACT
The genome segment S5 of rice black-streaked dwarf virus (genus Fijivirus, family Reoviridae) is functionally bicistronic in infected plants. It has a conserved second ORF (P5-2) partially overlapping the major ORF in a different reading frame, but its function remains unknown. P5-2 was detected in infected plants, but not in purified viral particles by Western blotting, indicating that it is a non-structural protein. In immunoelectron microscopy, polyclonal antibodies against P5-2 specifically labelled chloroplasts of infected rice plants. When P5-2 fused with green fluorescent protein was transiently expressed in leaves of Nicotiana benthamiana, fluorescence was also co-localized with chloroplasts. Experiments with deletion mutants of P5-2 showed that its N-terminal part was responsible for its targeting to chloroplasts.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reoviridae
/
Vírion
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Cloroplastos
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Proteínas não Estruturais Virais
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Transporte Proteico
Idioma:
En
Revista:
Arch Virol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
China