Dual roles of autologous CD8+ T cells in hematopoietic progenitor cell mobilization and engraftment.
Transfusion
; 55(7): 1758-65; quiz 1757, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25757596
ABSTRACT
BACKGROUND:
Poor marrow cellularity alone cannot explain poor hematopoietic progenitor cell (HPC) mobilization. This study assessed the role of CD8+ T cells in HPC cell mobilization and engraftment. STUDY DESIGN ANDMETHODS:
Mobilization and engraftment were assessed in 192 autologous HPC donors. CD34+, CD4+, and CD8+ T-cell contents in apheresis products were evaluated. Using a chemotaxis assay, we assessed the effect of purified autologous CD8+ T cells from low and high mobilizers on HPC migration from high to low stromal cell-derived factor (SDF-1α) concentration gradients. We also assessed CD8+ T-cell content association with days to neutrophil engraftment.RESULTS:
The median number of CD34+ cells/kg was 4.7 × 10(6) . Patients were categorized according to their total CD34+ cell collection quartile distribution into low, moderate, and high mobilizers. We found that HPC products from low mobilizers contained more CD8+ T cells than HPC products from moderate and high mobilizers. Chemotaxis assays showed depletion of CD8+ T cells enhances HPC mobilization independent of SDF-1α concentration. Neutrophil engraftment analysis showed that the higher the CD8+ T-cell content per unit CD34+ cell, the faster the rate of engraftment.CONCLUSION:
Our findings suggest CD8+ T cells inhibit HPC mobilization and facilitate homing and engraftment.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quimiotaxia
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Leucaférese
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Transplante de Células-Tronco Hematopoéticas
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Linfócitos T CD8-Positivos
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Mobilização de Células-Tronco Hematopoéticas
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Sobrevivência de Enxerto
Limite:
Adolescent
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Adult
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Aged
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Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
Transfusion
Ano de publicação:
2015
Tipo de documento:
Article