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Genomes of 'Candidatus Liberibacter solanacearum' Haplotype A from New Zealand and the United States Suggest Significant Genome Plasticity in the Species.
Thompson, Sarah M; Johnson, Chris P; Lu, Ashley Y; Frampton, Rebekah A; Sullivan, Kerry L; Fiers, Mark W E J; Crowhurst, Ross N; Pitman, Andrew R; Scott, Ian A W; Wen, Aimin; Gudmestad, Neil C; Smith, Grant R.
Afiliação
  • Thompson SM; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Johnson CP; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Lu AY; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Frampton RA; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Sullivan KL; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Fiers MW; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Crowhurst RN; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Pitman AR; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Scott IA; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Wen A; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Gudmestad NC; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
  • Smith GR; First, third, fourth, fifth, sixth, eighth, ninth, and twelfth authors: The New Zealand Institute for Plant & Food Research Limited, Lincoln 7608, New Zealand; first, third, fourth, fifth, eighth, ninth, and twelfth authors: Plant Biosecurity Cooperative Research Centre, Canberra, ACT 2617, Aust
Phytopathology ; 105(7): 863-71, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25822188
ABSTRACT
'Candidatus Liberibacter solanacearum' contains two solanaceous crop-infecting haplotypes, A and B. Two haplotype A draft genomes were assembled and compared with ZC1 (haplotype B), revealing inversion and relocation genomic rearrangements, numerous single-nucleotide polymorphisms, and differences in phage-related regions. Differences in prophage location and sequence were seen both within and between haplotype comparisons. OrthoMCL and BLAST analyses identified 46 putative coding sequences present in haplotype A that were not present in haplotype B. Thirty-eight of these loci were not found in sequences from other Liberibacter spp. Quantitative polymerase chain reaction (qPCR) assays designed to amplify sequences from 15 of these loci were screened against a panel of 'Ca. L. solanacearum'-positive samples to investigate genetic diversity. Seven of the assays demonstrated within-haplotype diversity; five failed to amplify loci in at least one haplotype A sample while three assays produced amplicons from some haplotype B samples. Eight of the loci assays showed consistent A-B differentiation. Differences in genome arrangements, prophage, and qPCR results suggesting locus diversity within the haplotypes provide more evidence for genetic complexity in this emerging bacterial species.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhizobiaceae / Genoma Bacteriano / Solanaceae País/Região como assunto: America do norte / Oceania Idioma: En Revista: Phytopathology Assunto da revista: BOTANICA Ano de publicação: 2015 Tipo de documento: Article
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhizobiaceae / Genoma Bacteriano / Solanaceae País/Região como assunto: America do norte / Oceania Idioma: En Revista: Phytopathology Assunto da revista: BOTANICA Ano de publicação: 2015 Tipo de documento: Article