[CRISPR/Cas system for genome editing in pluripotent stem cells].
Tsitologiia
; 57(1): 19-30, 2015.
Article
em Ru
| MEDLINE
| ID: mdl-25872372
ABSTRACT
Genome editing systems based on site-specific nucleases became very popular for genome editing in modern bioengineering. Human pluripotent stem cells provide a unique platform for genes function study, disease modeling, and drugs testing. Consequently, technology for fast, accurate and well controlled genome manipulation is required. CRISPR/Cas (clustered regularly interspaced short palindromic repeat/CRISPR-associated) system could be employed for these purposes. This system is based on site-specific programmable nuclease Cas9. Numerous advantages of the CRISPR/Cas system and its successful application to human stem cells provide wide opportunities for genome therapy and regeneration medicine. In this publication, we describe and compare the main genome editing systems based on site-specific programmable nucleases and discuss opportunities and perspectives of the CRISPR/Cas system for application to pluripotent stem cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Engenharia Genética
/
Genoma Humano
/
Células-Tronco Pluripotentes
/
Endonucleases
/
Sistemas CRISPR-Cas
Limite:
Humans
Idioma:
Ru
Revista:
Tsitologiia
Ano de publicação:
2015
Tipo de documento:
Article