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Subcutaneous fat transplantation alleviates diet-induced glucose intolerance and inflammation in mice.
Hocking, Samantha L; Stewart, Rebecca L; Brandon, Amanda E; Suryana, Eurwin; Stuart, Ella; Baldwin, Emily M; Kolumam, Ganesh A; Modrusan, Zora; Junutula, Jagath R; Gunton, Jenny E; Medynskyj, Michael; Blaber, Sinead P; Karsten, Elisabeth; Herbert, Benjamin R; James, David E; Cooney, Gregory J; Swarbrick, Michael M.
Afiliação
  • Hocking SL; Diabetes and Metabolism Division, Garvan Institute of Medical Research, 384 Victoria Street, Darlinghurst, 2010, Sydney, NSW, Australia.
Diabetologia ; 58(7): 1587-600, 2015 Jul.
Article em En | MEDLINE | ID: mdl-25899451
AIMS/HYPOTHESIS: Adipose tissue (AT) distribution is a major determinant of mortality and morbidity in obesity. In mice, intra-abdominal transplantation of subcutaneous AT (SAT) protects against glucose intolerance and insulin resistance (IR), but the underlying mechanisms are not well understood. METHODS: We investigated changes in adipokines, tissue-specific glucose uptake, gene expression and systemic inflammation in male C57BL6/J mice implanted intra-abdominally with either inguinal SAT or epididymal visceral AT (VAT) and fed a high-fat diet (HFD) for up to 17 weeks. RESULTS: Glucose tolerance was improved in mice receiving SAT after 6 weeks, and this was not attributable to differences in adiposity, tissue-specific glucose uptake, or plasma leptin or adiponectin concentrations. Instead, SAT transplantation prevented HFD-induced hepatic triacylglycerol accumulation and normalised the expression of hepatic gluconeogenic enzymes. Grafted fat displayed a significant increase in glucose uptake and unexpectedly, an induction of skeletal muscle-specific gene expression. Mice receiving subcutaneous fat also displayed a marked reduction in the plasma concentrations of several proinflammatory cytokines (TNF-α, IL-17, IL-12p70, monocyte chemoattractant protein-1 [MCP-1] and macrophage inflammatory protein-1ß [ΜIP-1ß]), compared with sham-operated mice. Plasma IL-17 and MIP-1ß concentrations were reduced from as early as 4 weeks after transplantation, and differences in plasma TNF-α and IL-17 concentrations predicted glucose tolerance and insulinaemia in the entire cohort of mice (n = 40). In contrast, mice receiving visceral fat transplants were glucose intolerant, with increased hepatic triacylglycerol content and elevated plasma IL-6 concentrations. CONCLUSIONS/INTERPRETATION: Intra-abdominal transplantation of subcutaneous fat reverses HFD-induced glucose intolerance, hepatic triacylglycerol accumulation and systemic inflammation in mice.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Intolerância à Glucose / Gordura Subcutânea / Inflamação Limite: Animals Idioma: En Revista: Diabetologia Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Austrália País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Intolerância à Glucose / Gordura Subcutânea / Inflamação Limite: Animals Idioma: En Revista: Diabetologia Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Austrália País de publicação: Alemanha