Combination of BMP-2-releasing gelatin/ß-TCP sponges with autologous bone marrow for bone regeneration of X-ray-irradiated rabbit ulnar defects.
Biomaterials
; 56: 18-25, 2015 Jul.
Article
em En
| MEDLINE
| ID: mdl-25934275
ABSTRACT
The objective of this study is to evaluate the feasibility of gelatin sponges incorporating ß-tricalcium phosphate (ß-TCP) granules (gelatin/ß-TCP sponges) to enhance bone regeneration at a segmental ulnar defect of rabbits with X-ray irradiation. After X-ray irradiation of the ulnar bone, segmental critical-sized defects of 20-mm length were created, and bone morphogenetic protein-2 (BMP-2)-releasing gelatin/ß-TCP sponges with or without autologous bone marrow were applied to the defects to evaluate bone regeneration. Both gelatin/ß-TCP sponges containing autologous bone marrow and BMP-2-releasing sponges enhanced bone regeneration at the ulna defect to a significantly greater extent than the empty sponges (control). However, in the X-ray-irradiated bone, the bone regeneration either by autologous bone marrow or BMP-2 was inhibited. When combined with autologous bone marrow, the BMP-2 exhibited significantly high osteoinductivity, irrespective of the X-ray irradiation. The bone mineral content at the ulna defect was similar to that of the intact bone. It is concluded that the combination of bone marrow with the BMP-2-releasing gelatin/ß-TCP sponge is a promising technique to induce bone regeneration at segmental bone defects after X-ray irradiation.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ulna
/
Regeneração Óssea
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Fosfatos de Cálcio
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Células da Medula Óssea
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Alicerces Teciduais
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Proteína Morfogenética Óssea 2
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Gelatina
Limite:
Animals
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Japão