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Therapeutic efficacy of artemisinin-loaded nanoparticles in experimental visceral leishmaniasis.
Want, Muzamil Yaqub; Islamuddin, Mohammad; Chouhan, Garima; Ozbak, Hani A; Hemeg, Hassan A; Dasgupta, Anjan Kumar; Chattopadhyay, Asoke Prasun; Afrin, Farhat.
Afiliação
  • Want MY; Parasite Immunology Laboratory, Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110062, India.
  • Islamuddin M; Parasite Immunology Laboratory, Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110062, India.
  • Chouhan G; Parasite Immunology Laboratory, Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110062, India.
  • Ozbak HA; Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, P.O. Box 344, Universities Road, Medina 30001, Saudi Arabia.
  • Hemeg HA; Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, P.O. Box 344, Universities Road, Medina 30001, Saudi Arabia.
  • Dasgupta AK; Department of Biophysics and Biochemistry, Ballygunge Science College, University of Calcutta, Kolkata 700019, India.
  • Chattopadhyay AP; Department of Chemistry, University of Kalyani, West Bengal 741235, India.
  • Afrin F; Parasite Immunology Laboratory, Department of Biotechnology, Jamia Hamdard (Hamdard University), New Delhi 110062, India; Department of Medical Laboratories Technology, Faculty of Applied Medical Sciences, Taibah University, P.O. Box 344, Universities Road, Medina 30001, Saudi Arabia. Electronic add
Colloids Surf B Biointerfaces ; 130: 215-21, 2015 Jun 01.
Article em En | MEDLINE | ID: mdl-25936561
Visceral leishmaniasis (VL) is a fatal vector-borne parasitic syndrome attributable to the protozoa of the Leishmania donovani complex. The available chemotherapeutic options are not ideal due to their potential toxicity, high cost and prolonged treatment schedule. In the present study, we conjectured the use of nano drug delivery systems for plant-derived secondary metabolite; artemisinin as an alternative strategy for the treatment of experimental VL. Artemisinin-loaded poly lactic co-glycolic acid (ALPLGA) nanoparticles prepared were spherical in shape with a particle size of 220.0±15.0 nm, 29.2±2.0% drug loading and 69.0±3.3% encapsulation efficiency. ALPLGA nanoparticles administered at doses of 10 and 20mg/kg body weight showed superior antileishmanial efficacy compared with free artemisinin in BALB/c model of VL. There was a significant reduction in hepatosplenomegaly as well as in parasite load in the liver (85.0±5.4%) and spleen (82.0±2.4%) with ALPLGA nanoparticles treatment at 20mg/kg body weight compared to free artemisinin (70.3±0.6% in liver and 62.7±3.7% in spleen). In addition, ALPLGA nanoparticle treatment restored the defective host immune response in mice with established VL infection. The protection was associated with a Th1-biased immune response as evident from a positive delayed-type hypersensitivity reaction, escalated IgG2a levels, augmented lymphoproliferation and enhancement in proinflammatory cytokines (IFN-γ and IL-2) with significant suppression of Th2 cytokines (IL-10 and IL-4) after in vitro recall, compared to infected control and free artemisinin treatment. In conclusion, our results advocate superior efficacy of ALPLGA nanoparticles over free artemisinin, which was coupled with restoration of suppressed cell-mediated immunity in animal models of VL.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania donovani / Artemisininas / Nanopartículas / Leishmaniose Visceral Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Índia País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Leishmania donovani / Artemisininas / Nanopartículas / Leishmaniose Visceral Idioma: En Revista: Colloids Surf B Biointerfaces Assunto da revista: QUIMICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Índia País de publicação: Holanda