Molecular diagnosis of toxoplasmosis: value of the buffy coat for the detection of circulating Toxoplasma gondii.
Diagn Microbiol Infect Dis
; 82(4): 289-91, 2015 Aug.
Article
em En
| MEDLINE
| ID: mdl-25957458
ABSTRACT
Early detection of Toxoplasma tachyzoites circulating in blood using PCR is recommended for immunosuppressed patients at high risk for disseminated toxoplasmosis. Using a toxoplasmosis mouse model, we show that the sensitivity of detection is higher using buffy coat isolated from a large blood volume than using whole blood for this molecular monitoring.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Parasitologia
/
Toxoplasma
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Toxoplasmose
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Reação em Cadeia da Polimerase
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Técnicas de Diagnóstico Molecular
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Buffy Coat
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
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Prognostic_studies
/
Screening_studies
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Diagn Microbiol Infect Dis
Ano de publicação:
2015
Tipo de documento:
Article