Characterization of the interactions between ß-amyloid peptide and the membranes of human SK-N-SH cells.
FEBS Lett
; 589(15): 1929-34, 2015 Jul 08.
Article
em En
| MEDLINE
| ID: mdl-26028312
ABSTRACT
Interaction of ß-amyloid peptide (Aß) with cell membranes is thought to be an initial step in Alzheimer's disease (AD). However, some data are controversial and the underlying mechanisms remain unclear. In this report, two populations of Aß were found in human SK-N-SH neuroblastoma cells. Notably, one of the Aß populations was tightly inserted into the plasma membrane whilst the other was only peripherally associated with it. Here we show that reducing membrane cholesterol decreased the number of membrane-embedded Aßs and increased the number of membrane-attached Aßs. We also found that cholesterol depletion inhibited Aß degradation and exacerbated Aß-mediated cytotoxicity. Our detailed and direct observations provide specific insights into the mechanism of Aß membrane-associated toxicity.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos beta-Amiloides
/
Neuroblastoma
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2015
Tipo de documento:
Article