Disruption of lig4 improves gene targeting efficiency in the oleaginous fungus Mortierella alpina 1S-4.

Kikukawa, Hiroshi; Sakuradani, Eiji; Ando, Akinori; Okuda, Tomoyo; Ochiai, Misa; Shimizu, Sakayu; Ogawa, Jun

Kikukawa, Hiroshi; Sakuradani, Eiji; Ando, Akinori; Okuda, Tomoyo; Ochiai, Misa; Shimizu, Sakayu; Ogawa, Jun.

Afiliação

Kikukawa H; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Sakuradani E; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Institute of Technology and Science, Tokushima University, 2-1 Minami-Josanjima, Tokushima 770-8506, Japan.
Ando A; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Okuda T; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
Ochiai M; Research Institute, Suntory Global Innovation Center Ltd., 1-1-1 Wakayamadai, Shimamoto-cho, Mishima-gun, Osaka 618-8503, Japan.
Shimizu S; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Department of Bioscience and Biotechnology, Faculty of Bioenvironmental Science, Kyoto Gakuen University, 1-1 Nanjo, Sogabe, Kameoka 621-8555, Japan.
Ogawa J; Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan; Research Unit for Physiological Chemistry, Kyoto University, Kitashirakawa-Oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan. Electronic address: ogawa@kais.kyoto-u

J Biotechnol ; 208: 63-9, 2015 Aug 20.

Article em En | MEDLINE | ID: mdl-26052021

Assuntos

DNA Ligases/genética; Proteínas Fúngicas/genética; Técnicas de Silenciamento de Genes; Marcação de Genes/métodos; Mortierella/genética; DNA Ligases/metabolismo; Proteínas Fúngicas/metabolismo; Mortierella/enzimologia

Palavras-chave

DNA ligase 4 (lig4); Gene targeting; Homologous recombination

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA Ligases / Proteínas Fúngicas / Marcação de Genes / Mortierella / Técnicas de Silenciamento de Genes Idioma: En Revista: J Biotechnol Assunto da revista: BIOTECNOLOGIA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Japão

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