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Molecular cloning, characterization and expression of the energy homeostasis-associated gene in piglet.
J Zhejiang Univ Sci B ; 16(6): 524-32, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-26055914
ABSTRACT
The energy homeostasis-associated (Enho) gene encodes a secreted protein, adropin, which regulates the expression of hepatic lipogenic genes and adipose tissue peroxisome proliferator-activated receptor γ, a major regulator of lipogenesis. In the present study, the porcine (Sus scrofa) homologue of the Enho gene, which was named pEnho, was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The gene sequence was submitted into the GenBank of NCBI, and the access number is GQ414763. The pEnho encodes a protein of 76 amino acids which shows 75% similarity to Homo sapiens adropin. The expression profile of pEnho in tissues (liver, muscle, anterior jejunum, posterior jejunum, and ileum) was determined by quantitative real-time RT-PCR. pEnho was localized on porcine chromosome 10 and no introns were found. In conclusion, pEnho was cloned and analysed with the aim of increasing knowledge about glucose and lipid metabolism in piglets and helping to promote the health and growth of piglets through adropin regulation.
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Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: MEDLINE Assunto principal: Suínos / Regulação da Expressão Gênica no Desenvolvimento Limite: Animais Idioma: Inglês Revista: J Zhejiang Univ Sci B Assunto da revista: Biologia / Medicina Ano de publicação: 2015 Tipo de documento: Artigo