[Expression of caspase-3 and HAX-1 after cerebral contusion in rat].
Fa Yi Xue Za Zhi
; 31(1): 7-10, 14, 2015 Feb.
Article
em Zh
| MEDLINE
| ID: mdl-26058125
ABSTRACT
OBJECTIVE:
To observe the expression pattern of caspase-3 and HCLS1-associated protein X-1 (HAX-1) at different time after cerebral contusion in rat, and explore the new method for estimating the injury interval.METHODS:
The cerebral contusion model was established using adult SD male rats. Then the rats were randomly allocated into 8 groups 2 h, 6 h, 12 h, 1 d, 3 d, and 7 d after cerebral contusion, sham-operation and normal control. Expression of caspase-3 and HAX-1 protein after cerebral contusion in rat was detected by Western blotting. Laser scanning confocal microscope was used to observe the number of HAX-1 positive cells and TUNEL-stained cells after cerebral contusion.RESULTS:
The expression of caspase-3 increased parallelly with the time after cerebral contusion and reached the peak value on 3 d. The expression of caspase-3 decreased gradually and still maintained a high level expression on 7 d (P < 0.05). The expression of HAX-1 positive cell went up after injury, and reached the peak value at 6 h (P < 0.05), then turned down gradually after 12 h and went out of detection after 3 d. The number of TUNEL-stained cells increased obviously at 2 h and reached the peak value on 3 d. The number of TUNEL-stained apoptotic cells decreased gradually and still maintained a high level expression on 7 d (P < 0.05).CONCLUSION:
The expression of caspase-3 and HAX-1 after cerebral contusion has time sequential regularity, which may provide new evidence for forensic diagnosis of cerebral contusion interval.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lesões Encefálicas
/
Proteínas de Transporte
/
Cerebelo
/
Caspase 3
Limite:
Animals
Idioma:
Zh
Revista:
Fa Yi Xue Za Zhi
Assunto da revista:
JURISPRUDENCIA
Ano de publicação:
2015
Tipo de documento:
Article