Neutral red retention time assay in determination of toxicity of nanoparticles.
Mar Environ Res
; 111: 158-61, 2015 Oct.
Article
em En
| MEDLINE
| ID: mdl-26065811
ABSTRACT
The neutral red retention time (NRRT) assay is useful for detecting decreased lysosomal membrane stability in haemocytes sampled from bivalves, a phenomenon often associated with exposure to environmental pollutants including nanomaterials. Bivalves are popular sentinel species in ecotoxicology and use of NRRT in study of species in the genus Mytilus is widespread in environmental monitoring. The NRRT assay has been used as an in vivo test for toxicity of carbon nanoparticles (Moore MN, Readman JAJ, Readman JW, Lowe DM, Frickers PE, Beesley A. 2009. Lysosomal cytotoxicity of carbon nanoparticles in cells of the molluscan immune system An in vivo study. Nanotoxicology. 3 (1), 40-45). We here report application of this assay adapted to a microtitre plate format to a panel of metal and metal oxide nanoparticles (2 ppm). This showed that copper, chromium and cobalt nanoparticles are toxic by this criterion while gold and titanium nanoparticles are not. As the former three nanoparticles are often reported to be cytotoxic while the latter two are thought to be non-cytotoxic, these data support use of NRRT as a general in vitro assay in nanotoxicology.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Poluentes Químicos da Água
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Monitoramento Ambiental
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Mytilus edulis
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Nanopartículas Metálicas
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Indicadores e Reagentes
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Vermelho Neutro
Limite:
Animals
Idioma:
En
Revista:
Mar Environ Res
Assunto da revista:
BIOLOGIA
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SAUDE AMBIENTAL
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TOXICOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Irlanda