"Cap-and-Catch" Purification for Enhancing the Quality of Libraries of DNA Conjugates.
ACS Comb Sci
; 17(7): 393-8, 2015 Jul 13.
Article
em En
| MEDLINE
| ID: mdl-26083096
ABSTRACT
The potential of DNA-encoded combinatorial libraries (DECLs) as tools for hit discovery crucially relies on the availability of methods for their synthesis at acceptable purity and quality. Incomplete reactions in the presence of DNA can noticeably affect the purity of DECLs and methods to selectively remove unreacted oligonucleotide-based starting products would likely enhance the quality of DECL screening results. We describe an approach to selectively remove unreacted oligonucleotide starting products from reaction mixtures and demonstrate its applicability in the context of acylation of amino-modified DNA. Following an amide bond forming reaction, we treat unreacted amino-modified DNAs with biotinylating reagents and isolate the corresponding biotinylated oligonucleotides from the reaction mixture by affinity capture on streptavidin-coated sepharose. This approach, which yields the desired DNA-conjugate at enhanced purity, can be applied both to reactions performed in solution and to procedures in which DNA is immobilized on an anion exchange solid support.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
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Técnicas de Química Combinatória
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Amidas
Idioma:
En
Revista:
ACS Comb Sci
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
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