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An actin-myosin-II interaction is involved in maintaining the contractile ring in fission yeast.
Takaine, Masak; Numata, Osamu; Nakano, Kentaro.
Afiliação
  • Takaine M; Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan masaktakaine@gmail.com knakano@biol.tsukuba.ac.jp.
  • Numata O; Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan.
  • Nakano K; Department of Biological Sciences, Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennohdai, Tsukuba, Ibaraki 305-8577, Japan masaktakaine@gmail.com knakano@biol.tsukuba.ac.jp.
J Cell Sci ; 128(15): 2903-18, 2015 Aug 01.
Article em En | MEDLINE | ID: mdl-26092938
The actomyosin-based contractile ring, which assembles at the cell equator, maintains its circularity during cytokinesis in many eukaryotic cells, ensuring its efficient constriction. Although consistent maintenance of the ring is one of the mechanisms underpinning cytokinesis, it has not yet been fully addressed. We here investigated the roles of fission yeast myosin-II proteins [Myo2 and Myo3 (also known as Myp2)] in ring maintenance during cytokinesis, with a focus on Myo3. A site-directed mutational analysis showed that the motor properties of Myo3 were involved in its accumulation in the contractile ring. The assembled ring was often deformed and not properly maintained under conditions in which the activities of myosin-II proteins localizing to the contractile ring were decreased, leading to inefficient cell division. Moreover, Myo3 appeared to form motile clusters on the ring. We propose that large assemblies of myosin-II proteins consolidate the contractile ring by continuously binding to F-actin in the ring, thereby contributing to its maintenance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schizosaccharomyces / Citoesqueleto de Actina / Cadeias Pesadas de Miosina / Miosina Tipo II / Proteínas de Schizosaccharomyces pombe / Citocinese Idioma: En Revista: J Cell Sci Ano de publicação: 2015 Tipo de documento: Article País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schizosaccharomyces / Citoesqueleto de Actina / Cadeias Pesadas de Miosina / Miosina Tipo II / Proteínas de Schizosaccharomyces pombe / Citocinese Idioma: En Revista: J Cell Sci Ano de publicação: 2015 Tipo de documento: Article País de publicação: Reino Unido