Electrochemical detection of indole alkaloids of Catharanthus roseus in high-performance liquid chromatography.
Analyst
; 114(10): 1229-31, 1989 Oct.
Article
em En
| MEDLINE
| ID: mdl-2619069
ABSTRACT
Hydrodynamic voltammograms for indole and five indole alkaloids with different amino functions were obtained in order to evaluate the applicability of high-performance liquid chromatography (HPLC) with coulometric detection to these compounds. With the exception of serpentine, which has a quaternary nitrogen in its structure, all the compounds were oxidised and gave net signals of greater than 25 nA pmol(-1) at potentials of between +0.2 and +0.85 V versus a solid Pd electrode in an acetate-buffered (pH 6.5) water-methanol-acetonitrile system. An HPLC assay for quantifying picomole amounts of catharanthine and ajmalicine in Catharanthus roseus cell culture samples is described.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas
/
Alcaloides de Vinca
/
Ioimbina
/
Alcaloides de Triptamina e Secologanina
/
Alcaloides
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Analyst
Ano de publicação:
1989
Tipo de documento:
Article