Your browser doesn't support javascript.
loading
Induction of autophagy through the activating transcription factor 4 (ATF4)-dependent amino acid response pathway in maternal skeletal muscle may function as the molecular memory in response to gestational protein restriction to alert offspring to maternal nutrition.
Wang, Huan; Wilson, Gabriel J; Zhou, Dan; Lezmi, Stéphane; Chen, Xiuwen; Layman, Donald K; Pan, Yuan-Xiang.
Afiliação
  • Wang H; Department of Food Science and Human Nutrition,College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
  • Wilson GJ; Division of Nutritional Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
  • Zhou D; Department of Food Science and Human Nutrition,College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
  • Lezmi S; Division of Nutritional Sciences, College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
  • Chen X; Department of Food Science and Human Nutrition,College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
  • Layman DK; Department of Food Science and Human Nutrition,College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
  • Pan YX; Department of Food Science and Human Nutrition,College of Agricultural, Consumer and Environmental Sciences, University of Illinois at Urbana-Champaign,905 South Goodwin Avenue,Urbana,IL61801,USA.
Br J Nutr ; 114(4): 519-32, 2015 Aug 28.
Article em En | MEDLINE | ID: mdl-26198178
ABSTRACT
The aim of the present study was to investigate the mechanistic basis of protein deficiency during pregnancy in mother that is transduced to offspring. To this end, timed-pregnant Sprague-Dawley rats were fed either a control (20 % of energy from protein) or low-protein (LP, 8 % of energy from protein) diet during gestation. Tissues were collected after delivery from rat dams, and skeletal muscle was collected at postnatal day 38 from the offspring. Quantitative RT-PCR and Western blot analyses were performed to determine mRNA and protein levels. Histological analysis was performed to evaluate myofibre size. LP dams gained significantly less weight during pregnancy, developed muscle atrophy, and had significantly lower circulating threonine and histidine levels than control dams. The mRNA expression of the well-known amino acid response (AAR) pathway-related target genes was increased only in the skeletal muscle of LP dams, as well as the protein expression levels of activating transcription factor 4 (ATF4) and phosphorylated eukaryotic translation initiation factor 2α (p-eIF2α). The mRNA expression of autophagy-related genes was significantly increased in the skeletal muscle of LP dams. Moreover, the mRNA expression of genes involved in both AAR and autophagy pathways remained elevated and was memorised in the muscle of LP offspring that consumed a post-weaning control diet. Additionally, the LP diet increased an autophagy marker, microtubule-associated proteins 1A/1B light chain 3B (LC3B) protein expression in the skeletal muscle of rat dams, consistent with the initiation of autophagy. The LP diet further increased ATF4 binding at the predicted regions of AAR and autophagy pathway-related genes. Increased binding of ATF4 unveils the crucial role of ATF4 in the activation of autophagy in response to protein restriction. Our data suggest that molecular changes in maternal muscle are memorised in the offspring long after gestational protein restriction, reinforcing the role of maternal signalling in programming offspring health.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Proteínas Alimentares / Músculo Esquelético / Dieta com Restrição de Proteínas / Fenômenos Fisiológicos da Nutrição Pré-Natal / Fator 4 Ativador da Transcrição / Aminoácidos Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Revista: Br J Nutr Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Proteínas Alimentares / Músculo Esquelético / Dieta com Restrição de Proteínas / Fenômenos Fisiológicos da Nutrição Pré-Natal / Fator 4 Ativador da Transcrição / Aminoácidos Tipo de estudo: Prognostic_studies Limite: Animals / Pregnancy Idioma: En Revista: Br J Nutr Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos