Observing the Hydration Layer of Trehalose with a Linked Molecular Terahertz Probe.
J Phys Chem Lett
; 5(11): 1845-9, 2014 Jun 05.
Article
em En
| MEDLINE
| ID: mdl-26273863
ABSTRACT
The terahertz (THz) absorption bands of biomolecular hydration layers are generally swamped by absorption from bulk water. Using the disaccharide trehalose, we show that this limitation can be overcome by attaching a molecular probe. By time-resolving the fluorescence shift of the probe, a local THz spectrum is obtained. From the dependence on temperature and H2O/D2O exchange, it is concluded that the trehalose hydration layer is being observed. The region of dynamic water perturbation by the disaccharide encompasses the probe and is therefore larger than the first two solvation layers.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Phys Chem Lett
Ano de publicação:
2014
Tipo de documento:
Article
País de afiliação:
Alemanha