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Enzyme function is regulated by its localization.
Gifford, Stacey M; Meyer, Pablo.
Afiliação
  • Gifford SM; IBM T. J. Watson Research Center, Yorktown Heights, NY 10598, United States.
  • Meyer P; IBM T. J. Watson Research Center, Yorktown Heights, NY 10598, United States. Electronic address: pmeyerr@us.ibm.com.
Comput Biol Chem ; 59 Pt B: 113-22, 2015 Dec.
Article em En | MEDLINE | ID: mdl-26278972
To better understand how enzyme localization affects enzyme activity we studied the cellular localization of the glycosyltransferase MurG, an enzyme necessary for cell wall synthesis at the spore during sporulation in the bacterium Bacillus subtilis. During sporulation MurG was gradually enriched to the membrane at the forespore and point mutations in a MurG helical domain disrupting its localization to the membrane caused severe sporulation defects, but did not affect localization nor caused detectable defects during exponential growth. We found that this localization is dependent on the phospholipid cardiolipin, as in strains where the cardiolipin-synthesizing genes were deleted, MurG levels were diminished at the forespore. Furthermore, in this cardiolipin-less strain, MurG localization during sporulation was rescued by external addition of purified cardiolipin. These results support localization as a critical factor in the regulation of proper enzyme function and catalysis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus subtilis / Proteínas da Membrana Bacteriana Externa / N-Acetilglucosaminiltransferases Idioma: En Revista: Comput Biol Chem Assunto da revista: BIOLOGIA / INFORMATICA MEDICA / QUIMICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus subtilis / Proteínas da Membrana Bacteriana Externa / N-Acetilglucosaminiltransferases Idioma: En Revista: Comput Biol Chem Assunto da revista: BIOLOGIA / INFORMATICA MEDICA / QUIMICA Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Reino Unido