Functional expression of a putative geraniol 8-hydroxylase by reconstitution of bacterially expressed plant CYP76F45 and NADPH-cytochrome P450 reductase CPR I from Croton stellatopilosus Ohba.

Sintupachee, Siriluk; Promden, Worrawat; Ngamrojanavanich, Nattaya; Sitthithaworn, Worapan; De-Eknamkul, Wanchai

Sintupachee, Siriluk; Promden, Worrawat; Ngamrojanavanich, Nattaya; Sitthithaworn, Worapan; De-Eknamkul, Wanchai.

Afiliação

Sintupachee S; Program in Biotechnology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand; Research Unit for Natural Product Biotechnology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Promden W; Research Unit for Natural Product Biotechnology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Division of General Science, Faculty of Education, Buriram Rajabhat University, Buriram 31000, Thailand.
Ngamrojanavanich N; Department of Chemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
Sitthithaworn W; Department of Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot University, Nakonnayok 26120, Thailand.
De-Eknamkul W; Research Unit for Natural Product Biotechnology, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand. Electronic address: dw

Phytochemistry ; 118: 204-15, 2015 10.

Article em En | MEDLINE | ID: mdl-26300313

Assuntos

Croton/enzimologia; Sistema Enzimático do Citocromo P-450/metabolismo; NADPH-Ferri-Hemoproteína Redutase/metabolismo; Monoterpenos Acíclicos; Sequência de Aminoácidos; Croton/genética; DNA Complementar/genética; Escherichia coli/genética; Filogenia; Folhas de Planta/metabolismo; Proteínas Recombinantes/metabolismo; Terpenos/análise

Palavras-chave

19-E-vallesamine; CYP76F45; Croton stellatopilosus Ohba; Euphorbiaceae; Functional expression; Geraniol-8-hydroxylase; Monoterpenoid indole alkaloid; P450 reductase type I

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: NADPH-Ferri-Hemoproteína Redutase / Croton / Sistema Enzimático do Citocromo P-450 Idioma: En Revista: Phytochemistry Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Tailândia

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