Minimizing technical variation during sample preparation prior to label-free quantitative mass spectrometry.
Anal Biochem
; 490: 14-9, 2015 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-26302362
Sample preparation is the crucial starting point to obtain high-quality mass spectrometry data and can be divided into two main steps in a bottom-up proteomics approach: cell/tissue lysis with or without detergents and a(n) (in-solution) digest comprising denaturation, reduction, alkylation, and digesting of the proteins. Here, some important considerations, among others, are that the reagents used for sample preparation can inhibit the digestion enzyme (e.g., 0.1% sodium dodecyl sulfate [SDS] and 0.5 M guanidine HCl), give rise to ion suppression (e.g., polyethylene glycol [PEG]), be incompatible with liquid chromatography-tandem mass spectrometry (LC-MS/MS) (e.g., SDS), and can induce additional modifications (e.g., urea). Taken together, all of these irreproducible effects are gradually becoming a problem when label-free quantitation of the samples is envisioned such as during the increasingly popular high-definition mass spectrometry (HDMS(E)) and sequential window acquisition of all theoretical fragment ion spectra (SWATH) data-independent acquisition strategies. Here, we describe the detailed validation of a reproducible method with sufficient protein yield for sample preparation without any known LC-MS/MS interfering substances by using 1% sodium deoxycholate (SDC) during both cell lysis and in-solution digest.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mapeamento de Peptídeos
/
Proteômica
/
Métodos Analíticos de Preparação de Amostras
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Proteínas de Neoplasias
Limite:
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Bélgica
País de publicação:
Estados Unidos