Proteomic analysis of cellular response induced by boron neutron capture reaction in human squamous cell carcinoma SAS cells.
Appl Radiat Isot
; 106: 213-9, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26302661
ABSTRACT
To understand the mechanism of cell death induced by boron neutron capture reaction (BNCR), we performed proteome analyses of human squamous tumor SAS cells after BNCR. Cells were irradiated with thermal neutron beam at KUR after incubation under boronophenylalanine (BPA)(+) and BPA(-) conditions. BNCR mainly induced typical apoptosis in SAS cells 24h post-irradiation. Proteomic analysis in SAS cells suggested that proteins functioning in endoplasmic reticulum, DNA repair, and RNA processing showed dynamic changes at early phase after BNCR and could be involved in the regulation of cellular response to BNCR. We found that the BNCR induces fragments of endoplasmic reticulum-localized lymphoid-restricted protein (LRMP). The fragmentation of LRMP was also observed in the rat tumor graft model 20 hours after BNCT treatment carried out at the National Nuclear Center of the Republic of Kazakhstan. These data suggest that dynamic changes of LRMP could be involved during cellular response to BNCR.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Bucais
/
Carcinoma de Células Escamosas
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Terapia por Captura de Nêutron de Boro
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Apoptose
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Proteômica
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Proteínas de Neoplasias
Limite:
Humans
Idioma:
En
Revista:
Appl Radiat Isot
Assunto da revista:
MEDICINA NUCLEAR
/
SAUDE AMBIENTAL
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Japão
País de publicação:
ENGLAND
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ESCOCIA
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GB
/
GREAT BRITAIN
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INGLATERRA
/
REINO UNIDO
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SCOTLAND
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UK
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UNITED KINGDOM