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Method with high-throughput screening potential for antioxidative substances using Escherichia coli biosensor katG'::lux.
Tienaho, Jenni; Sarjala, Tytti; Franzén, Robert; Karp, Matti.
Afiliação
  • Tienaho J; Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland; Natural Resources Institute Finland (Luke), Parkano Research Unit, Kaironiementie 15, FI-39700 Parkano, Finland. Electronic address: jenni.tienaho@tut.fi.
  • Sarjala T; Natural Resources Institute Finland (Luke), Parkano Research Unit, Kaironiementie 15, FI-39700 Parkano, Finland. Electronic address: tytti.sarjala@luke.fi.
  • Franzén R; Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland. Electronic address: robert.franzen@tut.fi.
  • Karp M; Department of Chemistry and Bioengineering, Tampere University of Technology, Korkeakoulunkatu 8, FI-33101 Tampere, Finland. Electronic address: matti.karp@tut.fi.
J Microbiol Methods ; 118: 78-80, 2015 Nov.
Article em En | MEDLINE | ID: mdl-26327540
ABSTRACT
A new method is described for the rapid real-time screening of antioxidative properties using a recombinant Escherichia coli DPD2511 biosensor. This microplate technique, without time-consuming pre-incubations and handling, has potential for a high-throughput search of bioactive compounds. Special emphasis was given to obtaining highly reliable and repeatable results.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Escherichia coli / Ensaios de Triagem em Larga Escala / Antioxidantes Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: J Microbiol Methods Ano de publicação: 2015 Tipo de documento: Article País de publicação: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Escherichia coli / Ensaios de Triagem em Larga Escala / Antioxidantes Tipo de estudo: Diagnostic_studies / Screening_studies Idioma: En Revista: J Microbiol Methods Ano de publicação: 2015 Tipo de documento: Article País de publicação: HOLANDA / HOLLAND / NETHERLANDS / NL / PAISES BAJOS / THE NETHERLANDS