The structure-forming role of magnesium pyrophosphate in the formation of DNA-containing microparticles during PCR.

Danilevich, V N; Machulin, A V; Sorokin, V V; Mulyukin, A L

Danilevich, V N; Machulin, A V; Sorokin, V V; Mulyukin, A L.

Afiliação

Danilevich VN; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow, 117997, Russia, dan@mx.ibch.ru.

Dokl Biochem Biophys ; 463: 259-63, 2015.

Article em En | MEDLINE | ID: mdl-26335826

ABSTRACT

ABSTRACT

This work is devoted to studying the mechanisms of formation of DNA-containing microparticles (MPs) during PCR. It was found that pyrophosphate, a byproduct of DNA synthesis, and magnesium cations are required for their formation, as evidenced by the results of biochemical and electron microscopy studies.

Assuntos

DNA/química; DNA/metabolismo; Difosfatos/metabolismo; Compostos de Magnésio/metabolismo; Microesferas; Reação em Cadeia da Polimerase; DNA/genética; Difosfatos/farmacologia; Cloreto de Magnésio/farmacologia; Compostos de Magnésio/farmacologia

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: DNA / Reação em Cadeia da Polimerase / Compostos de Magnésio / Difosfatos / Microesferas Idioma: En Revista: Dokl Biochem Biophys Assunto da revista: BIOFISICA / BIOQUIMICA Ano de publicação: 2015 Tipo de documento: Article

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