Simple in vitro assay of Arf GAPs and preparation of Arf proteins as substrates.
Methods Cell Biol
; 130: 69-80, 2015.
Article
em En
| MEDLINE
| ID: mdl-26360029
ABSTRACT
Defining the interaction of Arf GAPs with specific Arfs is important for understanding their functions in the endocytic system. Cell-based approaches have been valuable for identifying Arfs and Arf GAPs active in the endocytic compartment; however, the cell-based assays have some limitations in establishing relationships among the Arfs and ArfGAPs. Here we describe a simple in vitro assay that will provide a means for comparing Arfs as substrates and serve to complement cell-based studies.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fator 1 de Ribosilação do ADP
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Methods Cell Biol
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Estados Unidos