Nanobody-targeted E3-ubiquitin ligase complex degrades nuclear proteins.
Sci Rep
; 5: 14269, 2015 Sep 16.
Article
em En
| MEDLINE
| ID: mdl-26373678
ABSTRACT
Targeted protein degradation is a powerful tool in determining the function of specific proteins or protein complexes. We fused nanobodies to SPOP, an adaptor protein of the Cullin-RING E3 ubiquitin ligase complex, resulting in rapid ubiquitination and subsequent proteasome-dependent degradation of specific nuclear proteins in mammalian cells and zebrafish embryos. This approach is easily modifiable, as substrate specificity is conferred by an antibody domain that can be adapted to target virtually any protein.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Nucleares
/
Complexos Ubiquitina-Proteína Ligase
/
Ubiquitina-Proteína Ligases
/
Anticorpos de Domínio Único
Limite:
Animals
Idioma:
En
Revista:
Sci Rep
Ano de publicação:
2015
Tipo de documento:
Article