Structural heterogeneity of a human melanoma-associated antigen.
Cancer Res
; 49(2): 459-65, 1989 Jan 15.
Article
em En
| MEDLINE
| ID: mdl-2642740
ABSTRACT
The biosynthesis, structure, and topology of a melanoma-associated antigen, previously defined with the monoclonal antibody NKI/C-3 was studied. A polyclonal rabbit antiserum was raised against the antigen with a broader reactivity than the previously used monoclonal antibody NKI/C-3. The antigen was shown to consist of a single protein backbone to which two or three N-linked glycans were added cotranslationally. Extensive further heterogeneity was generated in the Golgi compartment and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with cathepsin D, which is a marker for lysosomal functions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Neoplasias
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1989
Tipo de documento:
Article