A disposable bacterial lysis cartridge (BLC) suitable for an in situ water-borne pathogen detection system.
Analyst
; 140(22): 7776-83, 2015 Nov 21.
Article
em En
| MEDLINE
| ID: mdl-26460197
ABSTRACT
We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min(-1) and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS).
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bacillus subtilis
/
Microbiologia da Água
/
Carga Bacteriana
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Analyst
Ano de publicação:
2015
Tipo de documento:
Article