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Direct detection of illicit drugs from biological fluids by desorption/ionization mass spectrometry with nanoporous silicon microparticles.
Guinan, T M; Kirkbride, P; Della Vedova, C B; Kershaw, S G; Kobus, H; Voelcker, N H.
Afiliação
  • Guinan TM; ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, Mawson Institute, Australia. nico.voelcker@unisa.edu.au.
Analyst ; 140(23): 7926-33, 2015 Dec 07.
Article em En | MEDLINE | ID: mdl-26502296
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) is a high throughput analytical technique capable of detecting low molecular weight analytes, including illicit drugs, and with potential applications in forensic toxicology as well as athlete and workplace testing, particularly for biological fluids (oral fluids, urine and blood). However, successful detection of illicit drugs using SALDI-MS often requires extraction steps to reduce the inherent complexity of biological fluids. Here, we demonstrate an all-in-one extraction and analytical system consisting of hydrophobically functionalized porous silicon microparticles (pSi-MPs) for affinity SALDI-MS of prescription and illicit drugs. This novel approach allows for the analysis of drugs from multiple biological fluids without sample preparation protocols. The effect of pSi-MP size, pore diameter, pore depth and functionalization on analytical performance is investigated. pSi-MPs were optimized for the rapid and high sensitivity detection of methadone, cocaine and 3,4-methylenedioxymethamphetamine (MDMA). This optimized system allowed extraction and detection of methadone from spiked saliva and clinical urine samples. Furthermore, by detecting oxycodone in additional clinical saliva and plasma samples, we were able to demonstrate the versatility of the pSi-MP SALDI-MS technique.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Silício / Líquidos Corporais / Drogas Ilícitas / Técnicas de Química Analítica / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Nanopartículas Tipo de estudo: Diagnostic_studies / Guideline Limite: Humans Idioma: En Revista: Analyst Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Austrália País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Silício / Líquidos Corporais / Drogas Ilícitas / Técnicas de Química Analítica / Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz / Nanopartículas Tipo de estudo: Diagnostic_studies / Guideline Limite: Humans Idioma: En Revista: Analyst Ano de publicação: 2015 Tipo de documento: Article País de afiliação: Austrália País de publicação: Reino Unido