Determination of nifurtimox in dog plasma by stable-isotope dilution LC-MS/MS.
Bioanalysis
; 7(21): 2777-87, 2015 Nov.
Article
em En
| MEDLINE
| ID: mdl-26564573
ABSTRACT
BACKGROUND:
Nifurtimox is a 5-nitrofuran derived antiprotozoal drug used to treat diseases caused by trypanosomes including Chagas' disease and sleeping sickness (African trypanosomiasis). Available methods for the determination of nifurtimox in plasma are tedious and of low sensitivity. For the first time, an isotope dilution HPLC/MS/MS method for the sensitive quantitation of nifurtimox down to 10.0 µg/l in plasma is described.RESULTS:
Protein precipitation was used for sample preparation. Samples were analyzed on a standard triple quadrupole tandem mass spectrometer. The validated concentration range covers 10.0 µg/l (LLOQ) to 5000 µg/l. Interassay accuracy and precision (%CV) ranged from 98.4 to 101%, and 2.61 to 10.1%, respectively.CONCLUSION:
The method consists of very simple sample preparation and provides unmatched sensitivity, high reproducibility and robustness enabling analysis of large sample numbers. Method performance met current guidelines on bioanalytical method validation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Cromatografia Líquida
/
Espectrometria de Massas em Tandem
/
Marcação por Isótopo
/
Nifurtimox
Limite:
Animals
Idioma:
En
Revista:
Bioanalysis
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Alemanha