Anticoagulant and antithrombotic activities of modified xylofucan sulfate from the brown alga Punctaria plantaginea.
Carbohydr Polym
; 136: 826-33, 2016 Jan 20.
Article
em En
| MEDLINE
| ID: mdl-26572418
ABSTRACT
Selectively and totally sulfated (1 â 3)-linked linear homofucans bearing â¼ 20 monosaccharide residues on average have been prepared from the branched xylofucan sulfate isolated from the brown alga Punctaria plantaginea. Anticoagulant and antithrombotic properties of the parent biopolymer and its derivatives were assessed in vitro. Highly sulfated linear fucan derivatives were shown to inhibit clot formation in APTT assay and ristocetin induced platelets aggregation, while the partially sulfated analogs were inactive. In the experiments with purified proteins, fucan derivatives with degree of sulfation of â¼ 2.0 were found to enhance thrombin and factor Xa inhibition by antithrombin III. The effect of sulfated fucans on thrombin inhibition, which was similar to those of heparinoid Clexane(®) (enoxaparin) and of a fucoidan from the brown alga Saccharina latissima studied previously, can be explained by the multicenter interaction and formation of a ternary complex thrombin-antithrombin III-polysaccharide. The possibility of such complexation was confirmed by computer docking study.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Polissacarídeos
/
Xilose
/
Inibidores da Agregação Plaquetária
/
Extratos Vegetais
/
Anticoagulantes
Tipo de estudo:
Clinical_trials
Limite:
Humans
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2016
Tipo de documento:
Article