The role of Arabidopsis glutathione transferase F9 gene under oxidative stress in seedlings.
Acta Biol Hung
; 66(4): 406-18, 2015 Dec.
Article
em En
| MEDLINE
| ID: mdl-26616373
ABSTRACT
Arabidopsis thaliana contains 54 soluble glutathione transferases (GSTs, EC 2.5.1.18), which are thought to play major roles in oxidative stress responses, but little is known about the function of individual isoenzymes. The role of AtGST phi 9 (GSTF9) in the salt- and salicylic acid response was investigated using 2-week-old Atgstf9 and wild type (Wt) plants. Atgstf9 mutants accumulated more ascorbic acid (AsA) and glutathione (GSH) and had decreased glutathione peroxidase (GPOX) activity under control conditions. Treatment of 2-week-old seedlings with 10â»7 M salicylic acid (SA) for 48 h resulted in elevated H2O2level and enhanced GST activity in Atgstf9 plants, 10â»5 M SA treatment enhanced the malondialdehyde and dehydroascorbate contents compared to Wt. 50 and 150 mM NaCl increased the GST activity, AsA and GSH accumulation in Atgstf9 seedlings more pronounced than in Wt plants. We found that the Atgstf9 mutants had altered redox homeostasis under control and stress conditions, in which elevated AsA and GSH levels and modified GST and GPOX activities may play significant role. The half-cell potential values calculated from the concentration of GSH and GSSG indicate that this GST isoenzyme has an important role in the salt stress response.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
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Estresse Oxidativo
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Proteínas de Arabidopsis
/
Glutationa Transferase
Idioma:
En
Revista:
Acta Biol Hung
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Hungria