Efficacy of antimicrobial solutions against polymicrobial root canal biofilm.
Int Endod J
; 50(1): 77-83, 2017 Jan.
Article
em En
| MEDLINE
| ID: mdl-26660494
ABSTRACT
AIM:
To evaluate the antibiofilm activity of 2.5% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), 2% alexidine (ALX) and 0.2% cetrimide (CTR) alone and in combination on mature polymicrobial root canal biofilms on human dentine using confocal laser scanning microscopy (CLSM).METHODOLOGY:
Twenty-eight human dentine specimens were infected for 21 days with microbial samples collected from infected root canals of three volunteers. Antibiofilm activity of the irrigating solutions was evaluated after 3 min of contact time under CLSM. For quantification purposes, bioimage_L software was used. The variables evaluated were the log10 of total biovolume (µm3 ) and percentage of live cells (green) population. Statistical analysis of both variables was performed using an anova test and a post hoc Duncan test to determine significant clusters amongst groups. The variable green population percentage was previously subjected to the normalized Anscombe transformation.RESULTS:
The NaOCl group had a total biovolume and percentage of live cells significantly lower than the other groups (P Ë 0.001). The addition of 0.2% CTR significantly increased the antimicrobial effect of 2% CHX (P Ë 0.001). There were no significant differences between 0.2% CTR, 2% ALX and the combination of both (P Ë 0.05).CONCLUSIONS:
Overall, 2.5% NaOCl dissolved and killed bacteria significantly more efficiently when used against polymicrobial mature biofilm on human dentine. Cetrimide improved the antimicrobial activity of chlorhexidine and alexidine.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biofilmes
/
Dentina
/
Antibacterianos
Limite:
Humans
Idioma:
En
Revista:
Int Endod J
Ano de publicação:
2017
Tipo de documento:
Article
País de afiliação:
Espanha