Endothelial-like nitric oxide synthase immunolocalization by using gold nanoparticles and dyes.
Biomed Opt Express
; 6(12): 4738-48, 2015 Dec 01.
Article
em En
| MEDLINE
| ID: mdl-26713190
ABSTRACT
Immunofluorescence is a biological technique that allows displaying the localization of the target molecule through a fluorescent microscope. We used a combination of gold nanoparticles and the fluorescein isothiocianate, FITC, as optical contrast agents for laser scanning confocal microscopy imaging to localize the endothelial-like nitric oxide synthase in skeletal muscle cells in a three-dimensional tissue phantom at the depth of 4µm. The FITC detected fluorescence intensity from gold-nanoparticles-labelled cells was brighter than the emission intensity from unlabelled cells.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Biomed Opt Express
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Itália