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Shade avoidance 6 encodes an Arabidopsis flap endonuclease required for maintenance of genome integrity and development.
Zhang, Yijuan; Wen, Chunhong; Liu, Songbai; Zheng, Li; Shen, Binghui; Tao, Yi.
Afiliação
  • Zhang Y; School of Life Sciences, Xiamen Plant Genetics Key Laboratory, Xiamen University, Xiamen 361102, China State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361102, China.
  • Wen C; School of Life Sciences, Xiamen Plant Genetics Key Laboratory, Xiamen University, Xiamen 361102, China.
  • Liu S; Department of Cancer Genetics and Epigenetics, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA College of Life Sciences, Zhejiang University, Hangzhou, China Suzhou Health College, Suzhou Key Laboratory of Biotechnology for Laboratory Medicine, Suzhou, 215009, Jiangsu Province, Chi
  • Zheng L; Department of Cancer Genetics and Epigenetics, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
  • Shen B; Department of Cancer Genetics and Epigenetics, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
  • Tao Y; School of Life Sciences, Xiamen Plant Genetics Key Laboratory, Xiamen University, Xiamen 361102, China State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen 361102, China yitao@xmu.edu.cn.
Nucleic Acids Res ; 44(3): 1271-84, 2016 Feb 18.
Article em En | MEDLINE | ID: mdl-26721386
Flap endonuclease-1 (FEN1) belongs to the Rad2 family of structure-specific nucleases. It is required for several DNA metabolic pathways, including DNA replication and DNA damage repair. Here, we have identified a shade avoidance mutant, sav6, which reduces the mRNA splicing efficiency of SAV6. We have demonstrated that SAV6 is an FEN1 homologue that shows double-flap endonuclease and gap-dependent endonuclease activity, but lacks exonuclease activity. sav6 mutants are hypersensitive to DNA damage induced by ultraviolet (UV)-C radiation and reagents that induce double-stranded DNA breaks, but exhibit normal responses to chemicals that block DNA replication. Signalling components that respond to DNA damage are constitutively activated in sav6 mutants. These data indicate that SAV6 is required for DNA damage repair and the maintenance of genome integrity. Mutant sav6 plants also show reduced root apical meristem (RAM) size and defective quiescent centre (QC) development. The expression of SMR7, a cell cycle regulatory gene, and ERF115 and PSK5, regulators of QC division, is increased in sav6 mutants. Their constitutive induction is likely due to the elevated DNA damage responses in sav6 and may lead to defects in the development of the RAM and QC. Therefore, SAV6 assures proper root development through maintenance of genome integrity.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Genoma de Planta / Proteínas de Arabidopsis / Endonucleases Flap Tipo de estudo: Prognostic_studies Idioma: En Revista: Nucleic Acids Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Genoma de Planta / Proteínas de Arabidopsis / Endonucleases Flap Tipo de estudo: Prognostic_studies Idioma: En Revista: Nucleic Acids Res Ano de publicação: 2016 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido