Your browser doesn't support javascript.
loading
Glycosylation characterization of therapeutic mAbs by top- and middle-down mass spectrometry.
Tran, Bao Quoc; Barton, Christopher; Feng, Jinhua; Sandjong, Aimee; Yoon, Sung Hwan; Awasthi, Shivangi; Liang, Tao; Khan, Mohd M; Kilgour, David P A; Goodlett, David R; Goo, Young Ah.
Afiliação
  • Tran BQ; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Barton C; MedImmune, LLC, Gaithersburg, MD, USA.
  • Feng J; MedImmune, LLC, Gaithersburg, MD, USA.
  • Sandjong A; MedImmune, LLC, Gaithersburg, MD, USA.
  • Yoon SH; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Awasthi S; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Liang T; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Khan MM; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Kilgour DP; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Goodlett DR; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
  • Goo YA; Department of Pharmaceutical Sciences, School of Pharmacy, University of Maryland, Baltimore, MD, USA.
Data Brief ; 6: 68-76, 2016 Mar.
Article em En | MEDLINE | ID: mdl-26793758
ABSTRACT
A reference monoclonal antibody IgG1 and a fusion IgG protein were analyzed by top- and middle-down mass spectrometry with multiple fragmentation techniques including electron transfer dissociation (ETD) and matrix-assisted laser desorption ionization in-source decay (MALDI-ISD) to investigate heterogeneity of glycosylated protein species. Specifically, glycan structure, sites, relative abundance levels, and termini structural conformation were investigated by use of Fourier transform ion cyclotron resonance (FT-ICR) or high performance liquid chromatography electrospray ionization (HPLC-ESI) linked to an Orbitrap. Incorporating a limited enzymatic digestion by immunoglobulin G-degrading enzyme Streptococcus pyogenes (IdeS) with MALDI-ISD analysis extended sequence coverage of the internal region of the proteins without pre-fractionation. The data in this article is associated with the research article published in Journal of Proteomics (Tran et al., 2015) [1].

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Data Brief Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Data Brief Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Estados Unidos