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Fibronectin-Containing Extracellular Vesicles Protect Melanocytes against Ultraviolet Radiation-Induced Cytotoxicity.
Bin, Bum-Ho; Kim, Dae-Kyum; Kim, Nan-Hyung; Choi, Eun-Jeong; Bhin, Jinhyuk; Kim, Sung Tae; Gho, Yong Song; Lee, Ai-Young; Lee, Tae Ryong; Cho, Eun-Gyung.
Afiliação
  • Bin BH; Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin, Republic of Korea.
  • Kim DK; Department of Life Sciences, POSTECH, Pohang, Republic of Korea.
  • Kim NH; Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
  • Choi EJ; Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin, Republic of Korea.
  • Bhin J; Department of Chemical Engineering, POSTECH, Pohang, Republic of Korea.
  • Kim ST; Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin, Republic of Korea.
  • Gho YS; Department of Life Sciences, POSTECH, Pohang, Republic of Korea.
  • Lee AY; Department of Dermatology, Dongguk University Ilsan Hospital, Goyang, Republic of Korea.
  • Lee TR; Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin, Republic of Korea. Electronic address: TRLee@amorepacific.com.
  • Cho EG; Bioscience Research Division, R&D Unit, AmorePacific Corporation, Yongin, Republic of Korea. Electronic address: egcho@amorepacific.com.
J Invest Dermatol ; 136(5): 957-966, 2016 05.
Article em En | MEDLINE | ID: mdl-26854492
ABSTRACT
Skin melanocytes are activated by exposure to UV radiation to secrete melanin-containing melanosomes to protect the skin from UV-induced damage. Despite the continuous renewal of the epidermis, the turnover rate of melanocytes is very slow, and they survive for long periods. However, the mechanisms underlying the survival of melanocytes exposed to UV radiation are not known. Here, we investigated the role of melanocyte-derived extracellular vesicles in melanocyte survival. Network analysis of the melanocyte extracellular vesicle proteome identified the extracellular matrix component fibronectin at a central node, and the release of fibronectin-containing extracellular vesicles was increased after exposure of melanocytes to UVB radiation. Using an anti-fibronectin neutralizing antibody and specific inhibitors of extracellular vesicle secretion, we demonstrated that extracellular vesicles enriched in fibronectin were involved in melanocyte survival after UVB radiation. Furthermore, we observed that in the hyperpigmented lesions of patients with melasma, the extracellular space around melanocytes contained more fibronectin compared with normal skin, suggesting that fibronectin is involved in maintaining melanocytes in pathological conditions. Collectively, our findings suggest that melanocytes secrete fibronectin-containing extracellular vesicles to increase their survival after UVB radiation. These data provide important insight into how constantly stimulated melanocytes can be maintained in pathological conditions such as melasma.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Fibronectinas / Vesículas Extracelulares / Melanócitos / Melanose Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Invest Dermatol Ano de publicação: 2016 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Raios Ultravioleta / Fibronectinas / Vesículas Extracelulares / Melanócitos / Melanose Tipo de estudo: Prognostic_studies Limite: Humans Idioma: En Revista: J Invest Dermatol Ano de publicação: 2016 Tipo de documento: Article