Optimizing Biomaterials for Tissue Engineering Human Bone Using Mesenchymal Stem Cells.
Plast Reconstr Surg
; 137(3): 854-863, 2016 Mar.
Article
em En
| MEDLINE
| ID: mdl-26910665
ABSTRACT
BACKGROUND:
Adequate biomaterials for tissue engineering bone and replacement of bone in clinical settings are still being developed. Previously, the combination of mesenchymal stem cells in hydrogels and calcium-based biomaterials in both in vitro and in vivo experiments has shown promising results. However, results may be optimized by careful selection of the material combination.METHODS:
ß-Tricalcium phosphate scaffolds were three-dimensionally printed with five different hydrogels collagen I, gelatin, fibrin glue, alginate, and Pluronic F-127. The scaffolds had eight channels, running throughout the entire scaffold, and macropores. Mesenchymal stem cells (2 × 10) were mixed with each hydrogel, and cell/hydrogel mixes were dispersed onto the corresponding ß-tricalcium phosphate/hydrogel scaffold and cultured under dynamic-oscillating conditions for 6 weeks. Specimens were harvested at 1, 2, 4, and 6 weeks and evaluated histologically, radiologically, biomechanically and, at 6 weeks, for expression of bone-specific proteins by reverse-transcriptase polymerase chain reaction. Statistical correlation analysis was performed between radiologic densities in Hounsfield units and biomechanical stiffness.RESULTS:
Collagen I samples had superior bone formation at 6 weeks as demonstrated by volume computed tomographic scanning, with densities of 300 HU, similar to native bone, and the highest compression values. Bone specificity of new tissue was confirmed histologically and by the expression of alkaline phosphatase, osteonectin, osteopontin, and osteocalcin. The bone density correlated closely with histologic and biomechanical testing results.CONCLUSION:
Bone formation is supported best by ß-tricalcium phosphate/collagen I hydrogel and mesenchymal stem cells in collagen I hydrogel. CLINICAL QUESTION/LEVEL OF EVIDENCE Therapeutic, V.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
Diferenciação Celular
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Engenharia Tecidual
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Alicerces Teciduais
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Células-Tronco Mesenquimais
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Plast Reconstr Surg
Ano de publicação:
2016
Tipo de documento:
Article