Decellularized extracellular matrix microparticles as a vehicle for cellular delivery in a model of anastomosis healing.
J Biomed Mater Res A
; 104(7): 1728-35, 2016 07.
Article
em En
| MEDLINE
| ID: mdl-26946064
ABSTRACT
Extracellular matrix (ECM) materials from animal and human sources have become important materials for soft tissue repair. Microparticles of ECM materials have increased surface area and exposed binding sites compared to sheet materials. Decellularized porcine peritoneum was mechanically dissociated into 200 µm microparticles, seeded with fibroblasts and cultured in a low gravity rotating bioreactor. The cells avidly attached and maintained excellent viability on the microparticles. When the seeded microparticles were placed in a collagen gel, the cells quickly migrated off the microparticles and through the gel. Cells from seeded microparticles migrated to and across an in vitro anastomosis model, increasing the tensile strength of the model. Cell seeded microparticles of ECM material have potential for paracrine and cellular delivery therapies when delivered in a gel carrier. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A 104A 1728-1735, 2016.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
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Sistemas de Liberação de Medicamentos
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Matriz Extracelular
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Microesferas
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Modelos Biológicos
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Biomed Mater Res A
Assunto da revista:
ENGENHARIA BIOMEDICA
Ano de publicação:
2016
Tipo de documento:
Article