Development and characterization of microsatellite markers for the oomyceta Aphanomyces euteiches.
Fungal Genet Biol
; 91: 1-5, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-26964907
ABSTRACT
Aphanomyces euteiches Drechsler is a serious pathogen of leguminous crops that causes devastating root rot of pea worldwide. Given that A. euteiches is a diploid organism, robust, codominant markers are needed for population genetics studies. We have developed and screened a microsatellite-enriched small-insert genomic library for identification of A. euteiches SSR containing sequences. Fourteen out of the 48 primer pairs designed to amplify SSR, produced unambiguous polymorphic products in our test population of 94 isolates. The number of alleles at each locus ranged from one to four. The identification of new markers would enhance the ability to evaluate the genetic structure of A. euteiches populations, and pathogen evolution.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pisum sativum
/
Repetições de Microssatélites
/
Aphanomyces
Idioma:
En
Revista:
Fungal Genet Biol
Assunto da revista:
GENETICA
/
MICROBIOLOGIA
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
França