Your browser doesn't support javascript.
loading
Intra-articular injection of synovial mesenchymal stem cells improves cartilage repair in a mouse injury model.
Mak, J; Jablonski, C L; Leonard, C A; Dunn, J F; Raharjo, E; Matyas, J R; Biernaskie, J; Krawetz, R J.
Afiliação
  • Mak J; McCaig Institute for Bone &Joint Health, University of Calgary, Calgary, AB, Canada.
  • Jablonski CL; McCaig Institute for Bone &Joint Health, University of Calgary, Calgary, AB, Canada.
  • Leonard CA; McCaig Institute for Bone &Joint Health, University of Calgary, Calgary, AB, Canada.
  • Dunn JF; University of Calgary, Department of Surgery, Calgary, AB, Canada.
  • Raharjo E; McCaig Institute for Bone &Joint Health, University of Calgary, Calgary, AB, Canada.
  • Matyas JR; University of Calgary, Department of Radiology, Calgary, AB, Canada.
  • Biernaskie J; Experimental Imaging Centre, University of Calgary, Calgary, AB, Canada.
  • Krawetz RJ; University of Calgary, Department of Comparative Biology and Experimental Medicine, Calgary, AB, Canada.
Sci Rep ; 6: 23076, 2016 Mar 17.
Article em En | MEDLINE | ID: mdl-26983696
Controversy remains whether articular cartilage has an endogenous stem/progenitor cell population, since its poor healing capacity after injury can lead to diseases such as osteoarthritis. In the joint environment there are mesenchymal stem/progenitor cells (MSCs) in the synovial membrane and synovial fluid that can differentiate into cartilage, but it is still under debate if these cells contribute to cartilage repair in vivo. In this study, we isolated a Sca-1 positive, chondrogenesis capable population of mouse synovial MSCs from C57BL6 and MRL/MpJ "super-healer" strains. Intra-articular injection of Sca-1 + GFP + synovial cells from C57BL6 or MRL/MpJ into C57BL6 mice following cartilage injury led to increased cartilage repair by 4 weeks after injury. GFP expression was detected in the injury site at 2 weeks, but not 4 weeks after injury. These results suggest that synovial stem/progenitor cells, regardless of strain background, have beneficial effects when injected into an injured joint. MSCs derived from MRL/MpJ mice did not promote an increased repair capacity compared to MSCs derived from non-healing C57BL6 controls; however, MRL/MpJ MSCs were observed within the defect area at the time points examined, while C57BL6 MSCs were not.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membrana Sinovial / Cartilagem Articular / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Canadá País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Membrana Sinovial / Cartilagem Articular / Células-Tronco Mesenquimais Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Canadá País de publicação: Reino Unido