Effect of hyaluronic acid on chondrocyte apoptosis.
Acta Ortop Bras
; 23(2): 90-3, 2015.
Article
em En
| MEDLINE
| ID: mdl-27069407
ABSTRACT
OBJECTIVE:
To determine the percentage of apoptotic cells in a contusion model of osteoarthritis (OA) and to assess whether intra-articular injection of high doses of hyaluronic acid (HA) immediately after trauma reduces chondrocyte apoptosis.METHODS:
Forty knees from adult rabbits were impacted thrice with a 1 kg block released through a 1 meter tall cylinder (29.4 Joules). Subsequently, 2 mL of HA was injected in one knee and 2 mL saline in the contra-lateral knee. Medication were administered twice a week for 30 days, when animals were sacrificed. Specimens were prepared for optical microscopy exam and terminal deoxynucleotidyl transferase end labeling assay (TUNEL).RESULTS:
The apoptosis rate in the contusion model was 68.01% (± 19.73%), a higher rate than previously described. HA significantly reduced the rate of apoptosis to 53.52% (± 18.09) (p <0.001).CONCLUSION:
Intra-articular HA administration started immediately after trauma reduces impact-induced chondrocyte apoptosis rates in rabbits.
Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Acta Ortop Bras
Ano de publicação:
2015
Tipo de documento:
Article
País de afiliação:
Brasil
País de publicação:
BR
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BRASIL
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BRASILE
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BRAZIL
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BRESIL