Uncoupling protein and its mRNA in brown adipose tissue of newborn rabbits.
Can J Physiol Pharmacol
; 67(1): 54-8, 1989 Jan.
Article
em En
| MEDLINE
| ID: mdl-2713755
ABSTRACT
Guanosine diphosphate binding to the uncoupling protein of isolated mitochondria of brown adipose tissue in newborn rabbits was measured as an index of thermogenic activity. The binding was 0.281 +/- 0.022 nmol GDP/mg mitochondrial protein at 1 day of age, 0.214 +/- 0.017 at 3 days, 0.428 +/- 0.038 at 5 days, and 0.208 +/- 0.016 at 7 days. The increase in binding between 3 and 7 days of age suggests that the brown fat has an increased thermogenic capacity at that age. In addition, the potential for synthesis of the uncoupling protein was investigated in 1- to 5-day-old newborn rabbits by probing the total cellular ribonucleic acid for the messenger that codes for uncoupling protein. The amount of uncoupling protein messenger was highest at 1 day of age and declined at least until 5 days of age. Because the amount of uncoupling protein messenger decreased as the GDP binding increased, the results suggest that either the initially translated uncoupling protein was unmasked at about 5 days of age or there was a delay in the incorporation of uncoupling protein into the mitochondrial inner membrane, or both.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Desacopladores
/
Tecido Adiposo Marrom
/
RNA Mensageiro
/
Proteínas de Transporte
/
Nucleotídeos de Guanina
/
Guanosina Difosfato
/
Proteínas de Membrana
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Revista:
Can J Physiol Pharmacol
Ano de publicação:
1989
Tipo de documento:
Article
País de afiliação:
Canadá