In vitro cytotoxicity, genotoxicity and antigenotoxicity assessment of Solanum lycocarpum hydroalcoholic extract.

ABSTRACT

CONTEXT Solanum lycocarpum A. St.-Hil. (Solanaceae), popularly known as 'fruta-do-lobo' (wolf fruit), 'lobeira' and 'jurubebão', is commonly used by native people of Central Brazil in powder form or as a hydroalcoholic extract for the management of diabetes and obesity and to decrease cholesterol levels. OBJECTIVE: The present study determines the possible cytotoxic, genotoxic and antigenotoxic activities of hydroalcoholic extract of the S. lycocarpum fruits (SL). MATERIALS AND METHODS: The clonogenic efficiency assay was used to determine the cytotoxicity. Three concentrations of SL (16, 32 and 64 µg/mL) were used for the evaluation of its genotoxic and antigenotoxic potential on V79 cells using the micronucleus and comet assays. In the antigenotoxicity assays, the cells were treated simultaneously with SL and the alkylating agent methyl methanesulphonate (MMS, 44 µg/mL for the micronucleus assay and 22 µg/mL for the comet assay) as an inducer of micronuclei and DNA damage. RESULTS: The results showed that SL was cytotoxic at concentrations up to 64 µg/mL. No significant differences in the rate of chromosome or DNA damage were observed between cultures treated with SL and the control group. In addition, the frequencies of micronuclei and DNA damage induced by MMS were significantly reduced after treatment with SL. The damage reduction percentage ranged from 68.1% to 79.2% and 12.1% to 16.5% for micronucleus and comet assays, respectively. DISCUSSION AND CONCLUSION: SL exerted no genotoxic effect and exhibited chemopreventive activity against both genomic and chromosome damage induced by MMS.