Atomic force microscopy observation and characterization of single virions and virus-like particles by nano-indentation.
Curr Opin Virol
; 18: 82-8, 2016 06.
Article
em En
| MEDLINE
| ID: mdl-27253691
Structure and function of viruses are intimately related, and one of the goals in virology is to elucidate the mechanisms behind this relation. A variety of research endeavours is focused on studying these mechanisms and a relatively new technique in this field is Atomic Force Microscopy (AFM). Using AFM virions and virus-like particles can be imaged and manipulated at the single particle level. Here we review recent AFM nano-indentations studies unveiling for instance the mechanics of capsid-genome interactions, morphological changes that drive viral maturation, capsid stabilizing factors and viral uncoating. We show that in an increasing amount of literature a clear link between mechanics and infectivity is observed, which not only provides us with new fundamental insights into virology, but also provides ways to improve virus-like particles for applications in nanomedicine and nanotechnology.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírion
/
Vírus
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Microscopia de Força Atômica
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Nanotecnologia
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Curr Opin Virol
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Holanda
País de publicação:
Holanda