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Atomic force microscopy observation and characterization of single virions and virus-like particles by nano-indentation.
Marchetti, M; Wuite, Gjl; Roos, W H.
Afiliação
  • Marchetti M; Natuur- en Sterrenkunde & LaserLab, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
  • Wuite G; Natuur- en Sterrenkunde & LaserLab, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands.
  • Roos WH; Natuur- en Sterrenkunde & LaserLab, Vrije Universiteit, De Boelelaan 1081, 1081 HV Amsterdam, The Netherlands; Moleculaire Biofysica, Zernike instituut, Rijksuniversiteit Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands. Electronic address: w.h.roos@rug.nl.
Curr Opin Virol ; 18: 82-8, 2016 06.
Article em En | MEDLINE | ID: mdl-27253691
Structure and function of viruses are intimately related, and one of the goals in virology is to elucidate the mechanisms behind this relation. A variety of research endeavours is focused on studying these mechanisms and a relatively new technique in this field is Atomic Force Microscopy (AFM). Using AFM virions and virus-like particles can be imaged and manipulated at the single particle level. Here we review recent AFM nano-indentations studies unveiling for instance the mechanics of capsid-genome interactions, morphological changes that drive viral maturation, capsid stabilizing factors and viral uncoating. We show that in an increasing amount of literature a clear link between mechanics and infectivity is observed, which not only provides us with new fundamental insights into virology, but also provides ways to improve virus-like particles for applications in nanomedicine and nanotechnology.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírion / Vírus / Microscopia de Força Atômica / Nanotecnologia Limite: Animals / Humans Idioma: En Revista: Curr Opin Virol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Holanda País de publicação: Holanda

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vírion / Vírus / Microscopia de Força Atômica / Nanotecnologia Limite: Animals / Humans Idioma: En Revista: Curr Opin Virol Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Holanda País de publicação: Holanda