Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma.
Methods Mol Biol
; 1434: 91-105, 2016.
Article
em En
| MEDLINE
| ID: mdl-27300533
ABSTRACT
Electrophoretic mobility shift assays (EMSA) are used to characterize interactions between nucleic acids and proteins in native conditions. This is based on the fact that the electrophoretic mobility of a nucleic acid becomes slower when it forms complexes with proteins. There are many different variants and applications of this methodology. In this chapter we describe a detailed EMSA protocol applied to the study of asthma.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Asma
/
Ensaio de Desvio de Mobilidade Eletroforética
/
Proteínas de Ligação a DNA
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2016
Tipo de documento:
Article
País de afiliação:
Espanha