Your browser doesn't support javascript.
loading
Soil drying procedure affects the DNA quantification of Lactarius vinosus but does not change the fungal community composition.
Castaño, Carles; Parladé, Javier; Pera, Joan; Martínez de Aragón, Juan; Alday, Josu G; Bonet, José Antonio.
Afiliação
  • Castaño C; Forest Bioengineering Solutions S.A., Ctra. de Sant Llorenç de Morunys, Km. 2, 25280, Solsona, Spain. carles.castanyo@fbs.cat.
  • Parladé J; Departament de Producció Vegetal i Ciència Forestal, Universitat de Lleida-AGROTECNIO, Av. Rovira Roure, 191, 25198, Lleida, Spain. carles.castanyo@fbs.cat.
  • Pera J; Protecció Vegetal Sostenible, IRTA, Centre de Cabrils, Ctra. Cabrils Km. 2, 08348, Cabrils, Barcelona, Spain.
  • Martínez de Aragón J; Protecció Vegetal Sostenible, IRTA, Centre de Cabrils, Ctra. Cabrils Km. 2, 08348, Cabrils, Barcelona, Spain.
  • Alday JG; Centre Tecnològic Forestal de Catalunya, CTFC-CEMFOR, Ctra. de Sant. Llorenç de Morunys Km. 2, 25280, Solsona, Spain.
  • Bonet JA; Departament de Producció Vegetal i Ciència Forestal, Universitat de Lleida-AGROTECNIO, Av. Rovira Roure, 191, 25198, Lleida, Spain.
Mycorrhiza ; 26(8): 799-808, 2016 Nov.
Article em En | MEDLINE | ID: mdl-27317489
ABSTRACT
Drying soil samples before DNA extraction is commonly used for specific fungal DNA quantification and metabarcoding studies, but the impact of different drying procedures on both the specific fungal DNA quantity and the fungal community composition has not been analyzed. We tested three different drying procedures (freeze-drying, oven-drying, and room temperature) on 12 different soil samples to determine (a) the soil mycelium biomass of the ectomycorrhizal species Lactarius vinosus using qPCR with a specifically designed TaqMan® probe and (b) the fungal community composition and diversity using the PacBio® RS II sequencing platform. Mycelium biomass of L. vinosus was significantly greater in the freeze-dried soil samples than in samples dried at oven and room temperature. However, drying procedures had no effect on fungal community composition or on fungal diversity. In addition, there were no significant differences in the proportions of fungi according to their functional roles (moulds vs. mycorrhizal species) in response to drying procedures. Only six out of 1139 operational taxonomic units (OTUs) had increased their relative proportions after soil drying at room temperature, with five of these OTUs classified as mould or yeast species. However, the magnitude of these changes was small, with an overall increase in relative abundance of these OTUs of approximately 2 %. These results suggest that DNA degradation may occur especially after drying soil samples at room temperature, but affecting equally nearly all fungi and therefore causing no significant differences in diversity and community composition. Despite the minimal effects caused by the drying procedures at the fungal community composition, freeze-drying resulted in higher concentrations of L. vinosus DNA and prevented potential colonization from opportunistic species.
Assuntos
Palavras-chave
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solo / Microbiologia do Solo / Basidiomycota / DNA Fúngico / Água / Micorrizas Idioma: En Revista: Mycorrhiza Assunto da revista: BIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Espanha
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Solo / Microbiologia do Solo / Basidiomycota / DNA Fúngico / Água / Micorrizas Idioma: En Revista: Mycorrhiza Assunto da revista: BIOLOGIA Ano de publicação: 2016 Tipo de documento: Article País de afiliação: Espanha