Remote Preconditioning on Rat Hepatic Ischemia-Reperfusion Injury Downregulated Bax and Cleaved Caspase-3 Expression.
Transplant Proc
; 48(4): 1247-50, 2016 May.
Article
em En
| MEDLINE
| ID: mdl-27320597
ABSTRACT
OBJECTIVE:
Hepatic ischemia-reperfusion injury (IRI) is considered a major cause of hepatic damage in liver surgery. The aim of this study was to investigate the effect of the remote ischemic perconditioning method on hepatic IRI in a rat model.METHODS:
Seventeen rats underwent hepatic IRI for 30 minutes followed by reperfusion, and were divided into 3 groups group I, only hepatic IRI (n = 5); group II, hepatic IRI with remote perconditioning (n = 7); and group III, hepatic IRI with remote postconditioning (n = 5).RESULTS:
For Bax/ß-actin, mean values of the 3 groups (±standard deviation) were 1.29 ± 0.26 (group I), 0.89 ± 0.15 (group II), and 1.02 ± 0.23 (group III). The level of Bax/ß-actin in group II was significantly lower than in group I (P < .01). The cleaved Caspase-3/ß-actin ratio for groups I, II, and III was 0.93 ± 0.22, 0.46 ± 0.16, and 0.63 ± 0.22, respectively. The level of cleaved Caspase-3/ß-actin in groups II and III were significantly lower than in group I (P < .01 and P < .05, respectively). The Bcl-2/ß-actin ratio for groups I, II, and III was 1.01 ± 0.09, 1.19 ± 0.39, and 1.20 ± 0.12, respectively. However, there were no significant difference between groups II and III and group I.CONCLUSIONS:
The remote perconditioning on rat hepatic IRI downregulated the Bax and cleaved Caspase-3 expression.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
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Precondicionamento Isquêmico
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Proteína X Associada a bcl-2
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Caspase 3
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Fígado
Limite:
Animals
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2016
Tipo de documento:
Article