Expression of Endoplasmic Reticulum-Mediated Stress Proteins in FK506-Treated T-Lymphocytes.
Transplant Proc
; 48(4): 1292-6, 2016 May.
Article
em En
| MEDLINE
| ID: mdl-27320607
ABSTRACT
BACKGROUND:
FK506-induced apoptotic endoplasmic reticulum (ER)-mediated stress protein expression was investigated in Jurkat human T-lymphocytes.METHODS:
The effect of FK506 on apoptosis and cell viability were examined. FK506-induced apoptosis was confirmed by nuclear fragmentation after DAPI staining. Expression of apoptotic ER-mediated stress proteins was examined by means of Western blotting of Grp78/BiP, Grp94, double-stranded RNA-dependent protein kinase (PKR)-like ER kinase (PERK), phosphor-PERK, CHOP/GADD153, and Bak. A flow cytometry analysis was performed after DAF-DA or DCF-DA staining. FK506-induced apoptosis was dose-dependent (10 nmol/L) and time-dependent (72 hours).RESULTS:
Grp78/BiP and Grp94 expressions were increased 36 hours after FK506 treatment. Increased phospho-PERK expression was observed 6 hours after FK506 treatment and peak activation of phospho-PERK was observed at 36 hours. CHOP/GADD153 expression was increased 48 hours after FK506 treatment. Expression of iNOS after FK506 treatment began to increase at 12 hours, peaked at 24 hours, and decreased after 36 hours.CONCLUSIONS:
From these results, we confirmed that FK506 induces apoptosis and acts dose- and time-dependently to decrease the viability of Jurkat cells through activation of apoptosis signaling and expression of apoptotic ER-mediated stress proteins.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tacrolimo
/
Estresse do Retículo Endoplasmático
/
Imunossupressores
Limite:
Humans
Idioma:
En
Revista:
Transplant Proc
Ano de publicação:
2016
Tipo de documento:
Article